A test kit for measuring total antioxidant capacity

Abstract

According to the studying this research, absorbance detection system for flow injection analysis system was constructed by using syringe pump so the problem of bubbles in the system was solved successfully. The principle of the test-kit based on pushing the solutions into the system using double two ml syringe pumps. The solutions were mixed at mixing coil then the solution of the sample was inserted automatically by using six-port-valve. The detector was built from light-emitting-diodes (LEDs) with the wavelength at 620 nm. The product solution in cuvette was exposure then the light energy was transferred to electric current by photo-diode. The electricity was changed to voltage to micro-controller. The absorbance was calculated. The results, absorbance and flow rate were controlled by utilization of Labview software. The absorbance of the samples from the developed system was compared to the commercial spectrophotometer. The mean absolute percentage error of the flow rate was 1.2%.