The potential development of Ficus hispida Linn use to traditional herbs and cancer prevention for generate knowledge and heritage of local wisdom

Abstract

The study used the bark, leaves and fruit of Ficus hispida Linn to separation by Maceration and sequential extraction method with hexane, ethyl acetate and methanol to crude extracts. Crude extracts were assessed for their antioxidant activities tested using DPPH radical scavenging assay, total phenolic contents tested using the Folin–Ciocalteu method, cytotoxic activity against oral cavity cancer (KB), human small cell lung cancer (NCI-H187) and breast cancer (MCF-7) cancer cell lines, tested using the resazurin microplate assay (REMA). The results showed that 1. Crude methanol extracts from the fruit exhibited free radical scavenging effect on the DPPH assay with IC50 value of 27 g/ml, with the total phenolic contents value of 102.39 mg GAE/g dw of standard BHT. 2. Crude hexane extracts from the bark showed highest inhibited the growth of Vibrio paraheamolyticus, crude ethyl acetate extracts from the bark showed highest inhibited the growth of Streptococcus mutans ATCC 27175 and crude methanol extracts from the leaves showed highest inhibited the growth of Vibrio cholerae, most with MIC value of 7.80 g/ml. 3. Crude ethyl acetate and methanol extracts from F. Hispida exhibited inhibitory effect against oral cavity cancer (KB), human small cell lung cancer (NCI-H187) and breast cancer (MCF-7) cancer cell lines. These data support traditional uses of F. Hispida in folk medicine