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dc.contributor.authorNicomrat, Duongruitaien_US
dc.contributor.authorดวงฤทัย นิคมรัฐen_US
dc.contributor.authorLakthandee, Manochen_US
dc.contributor.authorมาโนช หลักฐานดีen_US
dc.date.accessioned2020-01-17T04:34:21Z
dc.date.available2020-01-17T04:34:21Z
dc.date.issued2020-01-17
dc.identifier.urihttp://repository.rmutp.ac.th/handle/123456789/3002
dc.descriptionรายงานการวิจัย -- มหาวิทยาลัยเทคโนโลยีราชมงคลพระนคร, 2560en_US
dc.description.abstractThe research team has isolated the cellulase producing microorganisms which have capability in producing extracellular cellulase enzyme for cellulosic degradation from leftover agricultural waste materials such as fruits and vegetables. After cellulosic material degradation, the products mainly were sugar moiety being the starting materials for further alcohol fermentation and/or possibly being used in cultivation of common microorganisms. In the study, when different 49 bacterial isolates were tested for their cellulase activity on Carboxymethyl cellulose (CMC), two bacteria, Bacillus spp. CE08 and 49 were selected due to high cellulase activity and thus tested for the physical and chemical properties. Their 16S rDNA sequence comparisons were close to Bacillus sp. isolate obtained from the soil. Both isolates are common aerobic/facultative aerobic bacteria with the length of 1-3 μm, Gram positive, and preferring the fermentation of maltose and xylose to glucose while minimal degradation on sucrose and lactose contents. Their optimum digestibility was at 40°C, pH 4.5 for the broth having unpurified extracellular cellulase activity at 3-4 μg /mg. The enzyme could use the substrateCMC the best, but rice straw more slowly but better than grass. However, the enzyme displayed the least digestion effectiveness on cotton. Both of these isolates are expectedly applied in the biological degradation process substituting chemicals that cause pollution problems to the environment. Additionally, this is one promise way to bring value of agricultural waste to the community.en_US
dc.description.sponsorshipRajamangala University of Technology Phra Nakhonen_US
dc.language.isothen_US
dc.subjectProducing microorganismsen_US
dc.subjectAlcohol fermentationen_US
dc.subjectการคัดแยกเชื้อจุลินทรีย์en_US
dc.subjectการผลิตเอนไซม์เซลลูเลสen_US
dc.subjectการหมักแอลกอฮอลen_US
dc.titleThe Isolation of highly potential cellulase enzymes producing microorganisms from vegetable wastes for application in alcohol fermentationen_US
dc.title.alternativeการคัดแยกเชื้อจุลินทรีย์ที่มศักยภาพในการผลิตเอนไซม์เซลลูเลสจากผักเหลือทิ้งและเปลือกผลไม้ เพื่อใช้ในการหมักแอลกอฮอลen_US
dc.typeResearch Reporten_US
dc.contributor.emailauthorduongruithai.n@rmutp.ac.then_US
dc.contributor.emailauthormnl.dee@gmail.comen_US


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