Antimicrobial activity of endophytic fungi isolated from myrtaceae at kuankreng peatlands

Abstract

The purpose of this research was to study antimicrobial activity of endophytic fungi isolated from Myrtaceae ( Melaleuca cajeputi, Malaleuca leucadendra, Syzygium cumini L. and Rhodomyrtus tomentasa (Ait.) Hassk.) at Kuankreng Peatlands. A total of 124 isolates of endophytic fungi were obtained from all plant types. Three week old fermentation broths of endophytic fungi were tested for antimicrobial activity against pathogenic microorganisms using the agar well diffusion method. Forty- six isolates (37.1%) inhibited at least 1 type of pathogen. Fermentation broth and cells of active endophytes were further extracted with organic solvents and tested for MIC using the microdilution broth method. The results show that 156 of the total of 174 crude extracts (89.6%) produced MIC ? 200 g/mL. The lowest MIC for S. aureus ATCC25923, MRSA SK1, P. aeruginosa ATCC27853, C. albicans ATCC90028,C. neoformans ATCC90012 and M. gypseum were 128, 200, 200, 64, 200, 16 and 200 g/mL, respectively.