Evaluation of genetic variation of neolissochilus stracheyi wild populations in the nan river watershed using microsatellite dna markers

Abstract

This study evaluated the genetic diversity of two natural populations of Neolissochilus stracheyi in Nan River watershed, Amphoe Bokluea (BK) and Amphoe Mae Jarim (MJ). We analyzed microsatellite markers originally developed for Tor tambroides (Nguten et al., 2009). Three out of five microsatellite loci analyzed were polymorphic . Genetic variation within BK and MJ populations were not different. The number of allele per locus and allelic richness averaged over three loci was 2.33 and 2.67 for BK and MJ respectively. Observed heterozygosity value was 0.50 and 0.56 and the average expected heterozygosity was 0.41 and 0.53 for BK and MJ respectively. In addition, the two populations were genetically distinct (P<0.05) and geographic isolation of two locations may explain this genetic pattern.