Method development of gamma-oryzanol analysis in rice bran with environmental friendly solvent

Abstract

This research project aim to develop method for analysis of gamma-oryzanol in rice bran with environmental friendly solvent. The total content of gamma-oryzanol in rice bran was investigated using the combination between partial extraction method with ethyl lactate solvent and the study of factors on partial extraction. The parameters that effect the amount of gamma-oryzanol with partial extraction method consist of weight of rice bran, volume of solvent and extraction time were conducted. The result shown the maximum content of gamma-oryzanol that extracted with 50 milligrams of rice bran, 5 milliliters of ethyl lactate and 2 minutes of extraction time. The quantity of gamma-oryzanol in rice bran that observed using partial extraction method with ethyl lactate solvent and classical extraction method were 4.23 and 4.32 milligram per gram rice bran, respectively. The results from both extraction methods were well agreement. When the extracted rice bran was separated with High Performance Liquid Chromatography, the result shown the main compositions of extracted rice bran including cycloartenyl ferulate, 24-Methylene cycloartanyl ferulate and campestanyl ferulate. The develop method for analysis of gamma-oryzanol in rice bran using partial extraction with ethyl lactate solvent is easy, use only small apparatus in laboratory, cheap, short-time consuming and use environmental friendly solvent.